The role of neuronal antibodies in cryptogenic new-onset refractory status epilepticus.

Most cases with new-onset refractory status epilepticus (NORSE) remain cryptogenic despite extensive diagnostic work-up. The aim of this study was to analyse the etiology and clinical features of NORSE and investigate known or potentially novel autoantibodies in cryptogenic NORSE (cNORSE). We retrospectively assessed the medical records of adults with status epilepticus at a Swiss tertiary referral center between 2010 and 2021. Demographic, diagnostic, therapeutic and outcome parameters were characterized. We performed post-hoc screening for known or potentially novel autoantibodies including immunohistochemistry (IHC) on rat brain with CSF and serum samples of cNORSE. 20 patients with NORSE were identified. Etiologies included infections (n=4), Creutzfeld-Jakob disease (n=1), CASPR2 autoimmune encephalitis (n=1), and carotid artery stenosis with recurrent perfusion deficit (n=1). Thirteen cases (65%) were cryptogenic despite detailed evaluation. A posteriori IHC for neuronal autoantibodies yielded negative results in all available serum (n=11) and CSF (n=9) samples of cNORSE. Our results suggest that neuronal antibodies are unlikely to play a major role in the pathogenesis of cNORSE. Future studies should rather focus on other - especially T-cell- and cytokine-mediated - mechanisms of autoinflammation in this devastating disease, which is far too poorly understood so far.