Detection and tracking of cytoplasmic G-quadruplexes in live cells.
Lu-Si RaoLiang HaoLiu-Yi LiuYou-Liang ZengBing-Bing LiangWenting LiuZong-Wan MaoPublished in: Chemical communications (Cambridge, England) (2023)
A TTPP probe was developed to distinguish G-quadruplexes (G4s) from other nucleic acid topologies through longer fluorescence lifetimes and higher quantum yields. In fluorescence lifetime imaging microscopy, TTPP enabled the visualization of cytoplasmic G4s in live cells, and showed the potential to detect cell apoptosis and ferroptosis by tracking cytoplasmic G4s.
Keyphrases
- induced apoptosis
- nucleic acid
- single molecule
- cell cycle arrest
- high resolution
- cell death
- energy transfer
- signaling pathway
- cell proliferation
- molecular dynamics
- endoplasmic reticulum stress
- oxidative stress
- optical coherence tomography
- high throughput
- label free
- risk assessment
- living cells
- quantum dots
- climate change
- human health
- fluorescent probe