Involvement of nerve growth factor in mouse hippocampal neuronal cell line (HT22) differentiation and underlying role of DNA methyltransferases.
Ming ZhangHongxia ZhengXiaolu ZhangXiaoli TianShengdi XuYou LiuShuyuan JiangXiaolei LiuRui ShiKerui GongShaochun YanHe WangGuo ShaoZhanjun YangPublished in: Journal of toxicology and environmental health. Part A (2018)
DNA methylation is an epigenetic event involved in regulation of gene transcription during cell differentiation. DNA methyltransferases (DNMT) play a role in differentiation of neural stem cells into neurons. The aim of this study was to determine whether nerve growth factor (NGF) was involved in differentiation of mouse hippocampal neuronal cell line (HT22) as assessed by IncuCyte. Quantitative PCR and western blot were used to measure gene and protein expression of DNMT as well as the activity of DNMTs. Treatment with NGF was found to upregulate both gene and protein expressions as well as total activity of DNMTs in differentiating HT22 cells. Compared to undifferentiating cells, the percentage of differentiating cells at S phase increased significantly when incubated with NGF. In undifferentiated cells, NGF failed to induce gene and protein expressions and activity of DNMTs. Data demonstrate that differentiation of HT22 cells by exposure to NGF involve the activation of DNMTs pathway.
Keyphrases
- growth factor
- induced apoptosis
- dna methylation
- cell cycle arrest
- genome wide
- gene expression
- copy number
- oxidative stress
- endoplasmic reticulum stress
- machine learning
- cell death
- cerebral ischemia
- neural stem cells
- spinal cord
- cell proliferation
- spinal cord injury
- mass spectrometry
- genome wide identification
- contrast enhanced
- artificial intelligence
- blood brain barrier
- big data
- data analysis
- replacement therapy