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Nocardia polymerase chain reaction (PCR)-based assay performed on bronchoalveolar lavage fluid after lung transplantation: A prospective pilot study.

Julien CoussementDavid LebeauxNajla El BizriVincent ClaesMichel KohnenDeborah SteenselsIsabelle ÉtienneHélène SalordEmmanuelle BergeronVeronica Rodriguez-Nava
Published in: PloS one (2019)
In this prospective study, Nocardia PCR was positive on BAL fluid from one fourth of the LTRs. Nocardia PCR-based assays should be used with caution on respiratory samples from LTRs because of the possible detection of airway colonization using this technique. Larger studies are required to determine the usefulness of the Nocardia PCR-based assay in transplant recipients.
Keyphrases
  • real time pcr
  • high throughput
  • label free
  • sensitive detection