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A new tetracyclic saponin from Astragalus glycyphyllos L. and its neuroprotective and hMAO-B inhibiting activity.

Aleksandar ShkondrovIlina KrastevaFranz BucarOlaf KunertMagdalena Kondeva-BurdinaIliana Ionkova
Published in: Natural product research (2018)
A new tetracyclic saponin, 17(R),20(R)-3β,6α,16β-trihydroxycycloartanyl-23-carboxylic acid 16-lactone 3-O-β-D-glucopyranoside (1) together with one known flavonoid, camelliaside A (2) were isolated from the aerial parts of Astragalus glycyphyllos L. Their structures were determined by chemical, HRESIMS and NMR methods. On 6-hydroxydopamine in vitro model on isolated rat brain synaptosomes, compounds 1-2 had statistically significant neuroprotective activity similar to that of Silibinin, tested at 100 μM. Saponin 1 possessed the most prominent neuroprotective and antioxidant effects in this in vitro model. On human recombinant monoamine oxidase type B enzyme compound 1 displayed strong inhibiting activity, compared to Selegiline (1 μM). It could be concluded that the new epoxycycloartane saponin 1 could be a promising leading structure in respect of neurodegenerative diseases.
Keyphrases
  • cerebral ischemia
  • signaling pathway
  • magnetic resonance
  • oxidative stress
  • mass spectrometry
  • subarachnoid hemorrhage