[ 68 Ga]Ga-Ornibactin for Burkholderia cepacia complex Infection Imaging Using Positron Emission Tomography.
Katerina BendovaVladislav RaclavskyRadko NovotnyDominika LuptakovaMiroslav PopperZbynek NovyMarian HajduchMilos PetrikPublished in: Journal of medicinal chemistry (2023)
Bacteria from the Burkholderia cepacia complex are generally considered to be non-pathogenic to the healthy population. However, some of these species may cause serious nosocomial infections in immunocompromised patients; as such, it is essential to diagnose these infections rapidly so that adequate treatment can be initiated. We report here the use of a radiolabeled siderophore, ornibactin (ORNB), for positron emission tomography imaging. We successfully radiolabeled ORNB with gallium-68 with high radiochemical purity and proved that the resulting complex has optimal in vitro characteristics. In mice, the complex did not show excessive accumulation in organs and was excreted in the urine. We demonstrated that the [ 68 Ga]Ga-ORNB complex accumulates at the site of Burkholderia multivorans infection, including pneumonia, in two animal infection models. These results suggest that [ 68 Ga]Ga-ORNB is a promising tool for the diagnosis, monitoring, and evaluation of the therapeutic response to B. cepacia complex infection.
Keyphrases
- pet ct
- positron emission tomography
- computed tomography
- high resolution
- ejection fraction
- end stage renal disease
- metabolic syndrome
- staphylococcus aureus
- physical activity
- prognostic factors
- mass spectrometry
- escherichia coli
- peritoneal dialysis
- cystic fibrosis
- insulin resistance
- type diabetes
- pseudomonas aeruginosa
- photodynamic therapy