An enzyme-free DNA circuit for the amplified detection of Cd2+ based on hairpin probe-mediated toehold binding and branch migration.

Published in: Chemical communications (Cambridge, England) (2019)

An enzyme-free DNA circuit was designed for the amplified detection of Cd2+ based on hairpin probe-mediated toehold binding and branch migration. A Cd2+-specific aptamer was used to recognize Cd2+ and a G-quadruplex was used to report the detection signal. The assay is sensitive, with a detection limit of 5 pM.

Keyphrases

label free
loop mediated isothermal amplification
real time pcr
single molecule
nk cells
quantum dots
cell free
gold nanoparticles
high throughput
air pollution
dna binding
heavy metals
transcription factor