The pursuit of xanthenoid fluorophores with near-infrared-II emission for in vivo applications.

As fluorescence imaging in the second near-infrared window (NIR-II, 1000-1700 nm) has gained increasing attention, it is inevitable that NIR-II fluorophores, the cornerstone of NIR-II imaging, have come to the middle of the stage. NIR-II xanthenoid fluorophores with good stability, high brightness, and fluorescence adjustability are becoming popular. We here reviewed the recent progress of xanthenoid fluorophores with NIR-II emission for in vivo applications. Especially, we focus on the strategies used for longer wavelength and fluorescence regulation to construct OFF-ON or ratiometric NIR-II fluorescent probes.