A set of Arabidopsis genes involved in the accommodation of the downy mildew pathogen Hyaloperonospora arabidopsidis.
Martina Katharina RiedAline BanharaFang-Yu HwuAndreas BinderAndrea A GustCaroline HöfleRalph HückelhovenThorsten NürnbergerMartin ParniskePublished in: PLoS pathogens (2019)
The intracellular accommodation structures formed by plant cells to host arbuscular mycorrhiza fungi and biotrophic hyphal pathogens are cytologically similar. Therefore we investigated whether these interactions build on an overlapping genetic framework. In legumes, the malectin-like domain leucine-rich repeat receptor kinase SYMRK, the cation channel POLLUX and members of the nuclear pore NUP107-160 subcomplex are essential for symbiotic signal transduction and arbuscular mycorrhiza development. We identified members of these three groups in Arabidopsis thaliana and explored their impact on the interaction with the oomycete downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa). We report that mutations in the corresponding genes reduced the reproductive success of Hpa as determined by sporangiophore and spore counts. We discovered that a developmental transition of haustorial shape occurred significantly earlier and at higher frequency in the mutants. Analysis of the multiplication of extracellular bacterial pathogens, Hpa-induced cell death or callose accumulation, as well as Hpa- or flg22-induced defence marker gene expression, did not reveal any traces of constitutive or exacerbated defence responses. These findings point towards an overlap between the plant genetic toolboxes involved in the interaction with biotrophic intracellular hyphal symbionts and pathogens in terms of the gene families involved.
Keyphrases
- candida albicans
- genome wide
- gene expression
- cell death
- arabidopsis thaliana
- dna methylation
- gram negative
- copy number
- high glucose
- cell cycle arrest
- diabetic rats
- antimicrobial resistance
- induced apoptosis
- transcription factor
- drug induced
- reactive oxygen species
- high resolution
- multidrug resistant
- single cell
- cell proliferation
- signaling pathway
- peripheral blood
- protein kinase
- mass spectrometry
- plant growth
- ionic liquid
- single molecule
- pi k akt