Susceptibility of Mycobacterium tuberculosis Cytochrome bd Oxidase Mutants to Compounds Targeting the Terminal Respiratory Oxidase, Cytochrome c.

Atica MoosaDirk A LamprechtKriti AroraClifton E BarryHelena I M BoshoffThomas R IoergerAdrie J C SteynValerie MizrahiDigby Francis Warner

Published in: Antimicrobial agents and chemotherapy (2017)

We deleted subunits I (cydA) and II (cydB) of the Mycobacterium tuberculosis cytochrome bd menaquinol oxidase. The resulting ΔcydA and ΔcydAB mutants were hypersusceptible to compounds targeting the mycobacterial bc1 menaquinol-cytochrome c oxidoreductase and exhibited bioenergetic profiles indistinguishable from strains deficient in the ABC-type transporter, CydDC, predicted to be essential for cytochrome bd assembly. These results confirm CydAB and CydDC as potential targets for drugs aimed at inhibiting a terminal respiratory oxidase implicated in pathogenesis.

Keyphrases

mycobacterium tuberculosis
pulmonary tuberculosis
escherichia coli
signaling pathway
wild type
electron transfer
drug delivery
respiratory tract