A repeat length variation in myo-inositol monophosphatase gene contributes to seed size trait in chickpea.
Vikas DwivediSwarup Kumar ParidaDebasis ChattopadhyayPublished in: Scientific reports (2017)
Chickpea (Cicer arietinum L.) is the third most important food legume crop. Seed size is the most economically important trait for chickpea. To understand the genetic regulation of seed size in chickpea, the present study established a three-way association of CT repeat length variation of a simple sequence repeat (SSR) in myo-inositol monophosphatase gene (CaIMP) with seed weight and phytic acid content by large scale validation and genotyping in a set of genetically diverse germplasm accessions and two reciprocal intra-specific mapping populations. Germplasms and mapping individuals with CT repeat-length expansion in the 5' untranslated region of CaIMP exhibited a pronounced increase in CaIMP protein level, enzymatic activity, seed-phytate content and seed weight. A chickpea transient expression system demonstrated this repeat-length variation influenced the translation of CaIMP mRNA, apparently by facilitating translation initiation. Our analyses proposed that the SSR marker derived from 5' UTR of a CaIMP gene is a promising candidate for selection of seed size/weight for agronomic trait improvement of chickpea.
Keyphrases
- genome wide
- copy number
- body mass index
- physical activity
- dna methylation
- weight loss
- high resolution
- computed tomography
- magnetic resonance imaging
- binding protein
- weight gain
- genetic diversity
- climate change
- contrast enhanced
- image quality
- transcription factor
- small molecule
- nitric oxide
- risk assessment
- amino acid
- positron emission tomography
- mass spectrometry
- human health
- genome wide analysis