Group B Streptococcus transcriptome when interacting with brain endothelial cells.
Nadine VollmuthBailey E BridgersMadelyn L ArmstrongJacob F WoodAbigail R GildeaEric R EspinalThomas Alexander HoovenGiulia BarbieriAlexander J WestermannTill SauerweinKonrad U FoerstnerAlexandra Schubert-UnkmeirBrandon J KimPublished in: Journal of bacteriology (2024)
(GBS) meningitis remains the leading cause of neonatal meningitis. Research work has identified surface factors and two-component systems that contribute to GBS disruption of the blood-brain barrier (BBB). These discoveries often relied on genetic screening approaches. Here, we provide transcriptomic data describing how GBS changes its transcriptome when interacting with brain endothelial cells. Additionally, we have phenotypically validated these data by obtaining mutants of a select regulator that is highly down-regulated during infection and testing on our BBB model. This work provides the research field with a validated data set that can provide an insight into potential pathways that GBS requires to interact with the BBB and open the door to new discoveries.
Keyphrases
- endothelial cells
- blood brain barrier
- electronic health record
- single cell
- rna seq
- genome wide
- big data
- gene expression
- white matter
- resting state
- minimally invasive
- protein kinase
- data analysis
- high glucose
- biofilm formation
- dna methylation
- vascular endothelial growth factor
- escherichia coli
- candida albicans
- staphylococcus aureus