Ascorbic acid as a modulator of inflammatory response against Candida albicans .
Noala Vicensoto Moreira MilhanAline da Graça SampaioCristiane Yumi Koga-ItoAngela BruzzanitiPublished in: Future microbiology (2024)
Aim: To evaluate the behavior of oral keratinocytes in the presence of Vitamin C (Vit C) and its anti-inflammatory potential. Materials & methods: Oral keratinocytes were initially exposed to 0.1-2.5 mM of Vit C and the metabolic activity and cell migration were evaluated using MTS assay and Ibidi culture inserts, respectively. After, the cells were challenged with Candida albicans and inflammatory markers were analyzed by qPCR. Results: The treatment was not cytotoxic, and the highest concentrations increased the metabolic activity at 24 h. Vit C delayed the cell migration at 48 and 72 h. Interestingly, it downregulated the genes IL-8 and IL-1β . Conclusion: Vit C could be an interesting adjuvant to anti-fungal treatment due to its anti-inflammatory potential.
Keyphrases
- candida albicans
- cell migration
- anti inflammatory
- biofilm formation
- inflammatory response
- induced apoptosis
- early stage
- oxidative stress
- pseudomonas aeruginosa
- risk assessment
- combination therapy
- gene expression
- transcription factor
- cystic fibrosis
- staphylococcus aureus
- cell death
- cell cycle arrest
- replacement therapy
- wound healing
- signaling pathway
- endoplasmic reticulum stress