Antibacterial Activity and Mechanism of Oxidized Bacterial Nanocellulose with Different Carboxyl Content.

Oxidized bacterial nanocellulose (OBC) has been reported to prevent microbial growth, but its antibacterial characteristics and mechanism are still unclear. In this study, the antibacterial mechanism of OBC was explored by detecting and assessing the interaction of OBC with different carboxyl content on Staphylococcus aureus and Escherichia coli. The results showed that OBC had strong antibacterial activity and anti-biofilm activity against S. aureus and E. coli, which was positively correlated with the carboxyl content of OBC. After OBC treatment, the bacteria adhesion was inhibited, and the cell membrane was destroyed leading to increased permeability. Further investigation revealed that the concentration of c-di-GMP that induced biofilm formation was significantly decreased to 1.81 pmol/mg after OBC treatment. In addition, OBC inactivated mature biofilms, with inactivation rates up to 79.3%. This study suggested that OBC has excellent antibacterial and anti-adhesion properties, which can increase the cell membrane permeability and inhibit c-di-GMP formation. In addition, OBC also has a strong inactivation effect on mature biofilm, which can be used as an effective anti-biofilm agent. This article is protected by copyright. All rights reserved.