SELEX-discovered aptamer that inhibits cellular interleukin-17/interleukin-17 receptor interaction and antagonizes interleukin-17 signaling.
Arifur Rahman MunshiTong WangYukio TakamoriTakehiro AndoTakumi YokoyamaDaisuke FujiZhehao XuSanthana VediMizuki YamamotoKeita TsukamotoTakashi KawakamiPublished in: Bioscience, biotechnology, and biochemistry (2023)
This research is based on a systematic evolution of ligands by exponential enrichment (SELEX), also referred to as in vitro selection against the extracellular domain of human interleukin-17 receptor A (IL-17RA). Pull-down assay via quantitative polymerase chain reaction and chemiluminescence detection showed that the cloned RNA with the enriched sequence bound to human IL-17RA and inhibited the interaction between IL-17RA and human interleukin-17A (IL-17A). We also revealed that the newly discovered IL-17RA-binding RNA aptamer bound to cellular IL-17RA, inhibited the cellular IL-17RA/IL-17A interaction, and antagonized cellular IL-17A signaling.
Keyphrases
- rheumatoid arthritis
- endothelial cells
- disease activity
- gold nanoparticles
- ankylosing spondylitis
- sensitive detection
- high resolution
- interstitial lung disease
- systemic lupus erythematosus
- high throughput
- mass spectrometry
- transcription factor
- idiopathic pulmonary fibrosis
- binding protein
- dna binding
- quantum dots
- nucleic acid
- molecularly imprinted