A recognition-induced three-dimensional bipedal DNA walker for highly sensitive detection of APE1.
Qingyi LiuQiongdan ZhangYuting ZhangFanghong TianKang LongYupei YangWei WangCaiyun PengHuizhen WangPublished in: Analytical methods : advancing methods and applications (2024)
In contrast to the unipedal DNA walker, a bipedal DNA walker features a broader walking area and exhibits faster walking kinetics, leading to enhanced amplification efficiency. In this study, we designed a stochastic three-dimensional (3D) bipedal DNA walker, capable of navigating AuNP-based 3D tracks, driven by exonuclease III (Exo III). This detection system enables the linear detection of the non-invasive biomarker apurinic/apyrimidinic endonuclease 1 (APE1) activity across a range of 0 to 120 U per mL, with a detection limit of 0.03 U per mL. The platform not only offers a novel DNA walker for sensitive APE1 detection in cell lysate but also facilitates the precise assessment of NCA's inhibitory effect on APE1. This research holds promise for future screening of other potential APE1 inhibitors.
Keyphrases
- circulating tumor
- loop mediated isothermal amplification
- sensitive detection
- cell free
- single molecule
- nucleic acid
- label free
- real time pcr
- quantum dots
- magnetic resonance imaging
- magnetic resonance
- single cell
- high throughput
- circulating tumor cells
- current status
- endothelial cells
- dna damage
- risk assessment
- diabetic rats
- artificial intelligence
- dna repair
- neural network