Solid-binding peptides for immobilisation of thermostable enzymes to hydrolyse biomass polysaccharides.
Andrew CareKerstin PetrollEmily S Y GibsonPeter L BergquistAnwar SunnaPublished in: Biotechnology for biofuels (2017)
The current work introduced the construction of functional biocatalytic modules for the hydrolysis of simple and complex polysaccharides. This technology exploited a silica-binding SBP to mediate effectively the rapid and simple immobilisation of thermostable enzymes onto readily-available and inexpensive silica-based matrices. A conceptual application of biocatalytic modules consisting of single or multiple enzymes was validated by hydrolysing various hemicellulosic polysaccharides.