A Murine Model of Veno-Arterial Extracorporeal Membrane Oxygenation.
Mousa KharnafSpencer HogueZachariah WilkesJames A ReagorDaniel G LeinoBenjamin GourleyLeah RosenfeldtQing MaPrasad DevarajanJoseph S PalumboJames S TweddellFarhan ZafarPublished in: ASAIO journal (American Society for Artificial Internal Organs : 1992) (2022)
The mechanisms driving the pathologic state created by extracorporeal membrane oxygenation (ECMO) remain poorly defined. We developed the first complete blood-primed murine model of veno-arterial ECMO capable of maintaining oxygenation and perfusion, allowing molecular studies that are unavailable in larger animal models. Fifteen C57BL/6 mice underwent ECMO by cannulating the left common carotid artery and the right external jugular vein. The mean arterial pressure was measured through cannulation of the femoral artery. The blood-primed circuit functioned well. Hemodynamic parameters remained stable and blood gas analyses showed adequate oxygenation of the animals during ECMO over a 1-hour timeframe. A significant increase in plasma-free hemoglobin was observed following ECMO, likely secondary to hemolysis within the miniaturized circuit components. Paralleling clinical data, ECMO resulted in a significant increase in plasma levels of multiple proinflammatory cytokines as well as evidence of early signs of kidney and liver dysfunction. These results demonstrate that this novel, miniature blood-primed ECMO circuit represents a functional murine model of ECMO that will provide unique opportunities for further studies to expand our knowledge of ECMO-related pathologies using the wealth of available genetic, pharmacological, and biochemical murine reagents not available for other species.
Keyphrases
- extracorporeal membrane oxygenation
- acute respiratory distress syndrome
- respiratory failure
- mechanical ventilation
- healthcare
- blood pressure
- type diabetes
- magnetic resonance
- gene expression
- metabolic syndrome
- artificial intelligence
- adipose tissue
- deep learning
- insulin resistance
- ultrasound guided
- case control
- rectal cancer