Effect of Temperature and Humidity on Milk Urea Nitrogen Concentration.

This study investigated the effect of ambient temperature and humidity on milk urea nitrogen (MUN) concentration in Holstein cows. Meteorological data corresponding to the dates of milk sampling were collected over six years. A linear mixed-effects model including a random effect term for cow identification was used to assess whether temperature and humidity were predictive of MUN concentration. Age, days in milk, temperature humidity index (THI), ration, milk yield, parity and somatic cell count were also evaluated as main effects in the model. A general linear model including all variables as random effects was then fitted to assess the contribution of each variable towards the variability in MUN concentration. Maximum daily temperature and humidity on the sampling day were positively associated with MUN concentration, but their interaction term was negatively associated, indicating that their effects were not independent and additive. Variables that contributed the most to the variability of MUN concentration were dietary crude protein (21%), temperature (18%) and other factors (24%) that were not assessed in the model (error term). Temperature has a significant influence on urea nitrogen concentration and should therefore always be considered when urea nitrogen concentration data are used to make inferences about the dietary management of dairy cows.