A stability-indicating assay by reversed-phase high performance liquid chromatography method was developed and validated for the determination of sulthiame (STM). The chromatographic separation was achieved on a reversed-phase NovaPack C18 column and an isocratic mobile phase consisting of deionized water:methanol (70:30, v/v). The flow rate was 1.0 mL/min (ultraviolet detection at 210 nm). The STM was separated within 2.83 min. The linearity of the method was demonstrated in the range of 20.0-200.0 μg/mL and a coefficient of determination of r (2) = 0.9999. The limits of detection and quantification were 4.2 and 9.5 μg/mL, respectively. The intraday and interday precisions were less than 1%. Accuracy of the method ranged from 98.3% to 101.7%, with a relative standard deviation of <1%. STM was degraded by accelerated breakdown in alkaline, acidic, or oxidative stress conditions. This method allows accurate and reliable determination of STM for drug stability assay in pharmaceutical studies.
Keyphrases
- solid phase extraction
- high performance liquid chromatography
- simultaneous determination
- molecularly imprinted
- tandem mass spectrometry
- liquid chromatography
- oxidative stress
- high throughput
- mass spectrometry
- high resolution
- emergency department
- magnetic resonance imaging
- ionic liquid
- loop mediated isothermal amplification
- signaling pathway
- ischemia reperfusion injury
- case control
- diabetic rats
- ms ms