Appearance of microtubules at the cytokinesis to interphase transition in Arabidopsis thaliana.

Microtubule arrays drastically reorganize during the cell cycle to facilitate specific events. Many cells contain a centrosome that dictates the assembly and organization of microtubule arrays. However, plant cells and many others do not contain centrosomes or discrete microtubule organizing centers. In plants, microtubules nucleate and polymerize from gamma-tubulin-containing complexes in the interphase cell cortex. During plant cell division, microtubules nucleate near nuclei to form the mitotic spindle and plant-specific phragmoplast required for cytokinesis. Therefore, during the plant cell cycle, microtubule nucleation shifts from cell cortex to the perinuclear region. While it is unclear how this shift occurs, previous studies observed microtubules that appeared to extend from nuclei into the cortex as cells transitioned into interphase in small cells. These data led to the hypothesis that microtubule nucleation complexes move from the nuclear surface to the cortex at the transition from cytokinesis into interphase. Here we document GFP labeled microtubules in living plant cells during the transition from cytokinesis to interphase. We observed apparent groups of microtubules spanning between the nucleus and cell cortex in large, vacuolated epidermal leaf cells. We also observed microtubules in the cell cortex that appeared separate from perinuclear-associated microtubules. While these cortical microtubules were not always seen, when present they were apparent before cytokinesis was complete and/or before nuclear-associated microtubules were obvious. These data add to and deepen the knowledge of microtubule reorganization at this cell cycle transition.