Gold Half-Shell-Coated Paclitaxel-Loaded PLGA Nanoparticles for the Targeted Chemo-Photothermal Treatment of Cancer.
Jaime IbarraDavid Encinas-BasurtoMario AlmadaJosué E Juárez-OnofreMiguel Angel ValdezSilvia BarbosaPablo TaboadaPublished in: Micromachines (2023)
Conventional cancer therapies suffer from nonspecificity, drug resistance, and a poor bioavailability, which trigger severe side effects. To overcome these disadvantages, in this study, we designed and evaluated the in vitro potential of paclitaxel-loaded, PLGA-gold, half-shell nanoparticles (PTX-PLGA/Au-HS NPs) conjugated with cyclo(Arg-Gly-Asp-Phe-Lys) (cyRGDfk) as a targeted chemo-photothermal therapy system in HeLa and MDA-MB-231 cancer cells. A TEM analysis confirmed the successful gold half-shell structure formation. High-performance liquid chromatography showed an encapsulation efficiency of the paclitaxel inside nanoparticles of more than 90%. In the release study, an initial burst release of about 20% in the first 24 h was observed, followed by a sustained drug release for a period as long as 10 days, reaching values of about 92% and 49% for NPs with and without near infrared laser irradiation. In in vitro cell internalization studies, targeted nanoparticles showed a higher accumulation than nontargeted nanoparticles, possibly through a specific interaction of the cyRGDfk with their homologous receptors, the ανβ3 y ανβ5 integrins on the cell surface. Compared with chemotherapy or photothermal treatment alone, the combined treatment demonstrated a synergistic effect, reducing the cell viability to 23% for the HeLa cells and 31% for the MDA-MB-231 cells. Thus, our results indicate that these multifuncional nanoparticles can be considered to be a promising targeted chemo-photothermal therapy system against cancer.
Keyphrases
- cancer therapy
- drug delivery
- drug release
- photodynamic therapy
- cell cycle arrest
- papillary thyroid
- induced apoptosis
- high performance liquid chromatography
- combination therapy
- squamous cell
- cell surface
- locally advanced
- stem cells
- mass spectrometry
- squamous cell carcinoma
- dna damage
- bone marrow
- early onset
- pi k akt
- breast cancer cells
- radiation therapy
- high resolution
- high speed
- chemotherapy induced
- liquid chromatography
- bone regeneration