A wheel-like DNA nanosensor with background correction for analysis of miRNA-21 in living cells.

A silica ring-based ratiometric fluorescence nanosensing and imaging platform was established for the quantification of miRNA-21 with carbon dots and nucleic-functionalized gold nanoparticles inside and outside the ring, respectively. In the presence of miRNA-21, on one hand, the fluorescence emission signal of the originally quenched 6-carboxyfluorescein (FAM), which was labeled on the nucleic acid molecules that modified on the gold nanoparticles, was re-emitted. On the other hand, the fluorescence intensity of the carbon dots inside the ring remained essentially unchanged, which served to eliminate background interference. The ring rather than the shell well maintained the fluorescence of the carbon dots. The ratio of the recovered fluorescence intensity of FAM to the fluorescence intensity of the carbon dots was linearly correlated with the logarithm of miRNA-21 concentration. The linear range of the probe for miRNA detection was more than 3 orders of magnitude, and the detection limit was as low as 2.5 aM. The feasibility of the method for clinical application was verified by the quantitative analysis of intracellular miRNA.