Compact Microfluidic Platform with LED Light-Actuated Valves for Enzyme-Linked Immunosorbent Assay Automation.
Mireia Burdó-MasferrerMaría Díaz-GonzálezAna SanchisÁlvaro CallejaMaría-Pilar MarcoCésar Fernández-SánchezAntonio BaldiPublished in: Biosensors (2022)
Lab-on-a-chip devices incorporating valves and pumps can perform complex assays involving multiple reagents. However, the instruments used to drive these chips are complex and bulky. In this article, a new wax valve design that uses light from a light emitting diode (LED) for both opening and closing is reported. The valves and a pumping chamber are integrated in lab-on-a-foil chips that can be fabricated at low cost using rapid prototyping techniques. A chip for the implementation of enzyme-linked immunosorbent assays (ELISA) is designed. A porous nitrocellulose material is used for the immobilization of capture antibodies in the microchannel. A compact generic instrument with an array of 64 LEDs, a linear actuator to drive the pumping chamber, and absorbance detection for a colorimetric readout of the assay is also presented. Characterization of all the components and functionalities of the platform and the designed chip demonstrate their potential for assay automation.
Keyphrases
- high throughput
- light emitting
- low cost
- aortic valve
- single cell
- aortic valve replacement
- loop mediated isothermal amplification
- transcatheter aortic valve replacement
- aortic stenosis
- gold nanoparticles
- healthcare
- primary care
- patient reported outcomes
- heart failure
- sensitive detection
- hydrogen peroxide
- climate change
- risk assessment
- mass spectrometry
- human health
- real time pcr
- ejection fraction
- high density
- neural network