Characterisation of the transcriptome and proteome of SARS-CoV-2 reveals a cell passage induced in-frame deletion of the furin-like cleavage site from the spike glycoprotein.
Andrew D DavidsonMaia Kavanagh WilliamsonSebastian LewisDeborah ShoemarkMiles W CarrollKate J HeesomMaria ZambonJoanna EllisPhilip A LewisJulian A HiscoxDavid A MatthewsPublished in: Genome medicine (2020)
Detection of an apparently viable deletion in the furin cleavage site of the S glycoprotein, a leading vaccine target, shows that this and other regions of SARS-CoV-2 proteins may readily mutate. The furin site directs cleavage of the S glycoprotein into functional subunits during virus entry or exit and likely contributes strongly to the pathogenesis and zoonosis of this virus. Our data emphasises that the viral genome sequence should be carefully monitored during the growth of viral stocks for research, animal challenge models and, potentially, in clinical samples. Such variations may result in different levels of virulence, morbidity and mortality.
Keyphrases
- sars cov
- respiratory syndrome coronavirus
- dna binding
- single cell
- genome wide
- escherichia coli
- pseudomonas aeruginosa
- staphylococcus aureus
- gene expression
- high glucose
- electronic health record
- rna seq
- antimicrobial resistance
- biofilm formation
- bone marrow
- cell therapy
- transcription factor
- loop mediated isothermal amplification
- machine learning
- drug induced
- dna methylation
- endothelial cells
- deep learning
- coronavirus disease