A new apotirucallane-type protolimonoid from the leaves of Paramignya trimera .

This study presents a phytochemical analysis of the leaves of Paramignya trimera , revealing the isolation of a new apotirucallane-type protolimonoid, identified as 25- O -methyl-1,2-dihydroprotoxylocarpin D ( 1 ), along with two known compounds ( 2 and 3 ). The known compounds were identified as (20 S ,21 R ,23 R )-21,23-epoxy-7 α ,24,25-trihydroxy-21- O -methyl-3-oxoapotirucalla-14-ene ( 2 ) and 7 α ,24,25-trihydroxy-3-oxoapotirucalla-14-en-21,23-olide ( 3 ). The three apotirucallane-type protolimonoids ( 1 - 3 ) did not exhibit cytotoxicity against MCF-7 cells at a concentration of 100 µM. Interestingly, when MCF-7 cells were treated with compound 1 at various concentrations, a notable stimulatory response was observed, leading to a significant increase in cell viability, up to 127%.