A new approach with dried plasma spots: a rapid determination of antidepressants and antipsychotics by fast GC-MS.
Giovanna Cristiano de GouveiaBruno Pereira Dos SantosGabriela Ramos BorgesViviane Cristina SebbenSarah C W S E Franco de OliveiraTiago Franco de OliveiraPublished in: Bioanalysis (2023)
Background: The increasing prevalence of poisoning cases related to antidepressants and antipsychotics has raised concerns. Methods: To address this issue, a new adaptation of the dried plasma spot technique was developed using a 24-well plate and fast gas chromatography-mass spectrometry. The method involves the optimization of extraction variables and sample preparation, and was successfully validated. Results: The limits of quantitation ranged from 20 to 60 ng/ml, and accuracy ranged from 87.8% to 112.2%. The technique was applied to 102 human plasma samples from suspected poisoning cases, with positivity of 90.2%. Conclusion: This method provides a cheap, easy to implement and fast approach, making it ideal for toxicological emergency laboratories and promoting valuable support for healthcare professionals managing poisoning cases involving antidepressants and antipsychotics.
Keyphrases
- gas chromatography mass spectrometry
- major depressive disorder
- solid phase extraction
- molecularly imprinted
- public health
- emergency department
- healthcare
- mass spectrometry
- risk factors
- pulmonary embolism
- bipolar disorder
- tandem mass spectrometry
- liquid chromatography
- high resolution
- simultaneous determination
- quantum dots
- sensitive detection