Group B Streptococcus transcriptome when interacting with brain endothelial cells.
Nadine VollmuthBailey E BridgersMadelyn L ArmstrongJacob F WoodAbigail R GildeaEric R EspinalThomas Alexander HoovenGiulia BarbieriAlexander J WestermannTill SauerweinKonrad U FoerstnerAlexandra Schubert-UnkmeirBrandon J KimPublished in: Journal of bacteriology (2024)
(GBS) meningitis remains the leading cause of neonatal meningitis. Research work has identified surface factors and two-component systems that contribute to GBS disruption of the blood-brain barrier (BBB). These discoveries often relied on genetic screening approaches. Here, we provide transcriptomic data describing how GBS changes its transcriptome when interacting with brain endothelial cells. Additionally, we have phenotypically validated these data by obtaining mutants of a select regulator that is highly down-regulated during infection and testing on our BBB model. This work provides the research field with a validated data set that can provide an insight into potential pathways that GBS requires to interact with the BBB and open the door to new discoveries.
Keyphrases
- endothelial cells
- blood brain barrier
- electronic health record
- single cell
- rna seq
- genome wide
- big data
- resting state
- white matter
- transcription factor
- cerebral ischemia
- cerebrospinal fluid
- escherichia coli
- functional connectivity
- machine learning
- data analysis
- candida albicans
- multiple sclerosis
- vascular endothelial growth factor
- pseudomonas aeruginosa
- risk assessment
- artificial intelligence
- copy number
- climate change
- human health