MicroRNA-Catalyzed Cancer Therapeutics Based on DNA-Programmed Nanoparticle Complex.
Xucheng LuoZhi LiGanglin WangXuewen HeXiaoqin ShenQuanhong SunLi WangRenye YueNan MaPublished in: ACS applied materials & interfaces (2017)
The use of cancer-relevant microRNA molecules as endogenous drug release stimuli is promising for personalized cancer treatment yet remains a great challenge because of their low abundance. Herein, we report a new type of microRNA-catalyzed drug release system based on DNA-programmed gold nanoparticle (GNP)-quantum dot (QD) complex. We show that a trace amount of miRNA-21 molecules could specifically catalyze the disassembly of doxorubicin (Dox)-loaded GNP-QDs complex through entropy driven process, during which the Dox-intercalating sites are destructed for drug release. This catalytic reaction could proceed both in fixed cells and live cells with miRNA-21 overexpression. Dox molecules could be efficiently released in the cells and translocate to cell nuclei. QD photoluminescence is simultaneously activated during catalytic disassembly process, thus providing a reliable feedback for microRNA-triggered drug release. The GNP-QDs-Dox complex exhibits much higher drug potency than free Dox molecules, and therefore represents a promising platform for accurate and effective cancer cell treatment.
Keyphrases
- drug release
- drug delivery
- induced apoptosis
- cell cycle arrest
- papillary thyroid
- endoplasmic reticulum stress
- cancer therapy
- oxidative stress
- single molecule
- emergency department
- squamous cell carcinoma
- cell death
- cell free
- quantum dots
- high throughput
- single cell
- room temperature
- transcription factor
- high resolution
- young adults
- mass spectrometry
- microbial community
- bone marrow
- drug induced
- lymph node metastasis
- ionic liquid