Development of a method for the determination of 8-iso-PGF2α in sheep and goat plasma using solid-phase microextraction and ultra-performance liquid chromatography/tandem mass spectrometry.
Gabriela Rodríguez PatiñoMiriam Aide Castillo RodríguezJacinto Efren Ramírez BribiescaPatricia Ramírez NogueraMaría Eugenia Gonsebatt BonaparteRaquel Lopez-ArellanoPublished in: Rapid communications in mass spectrometry : RCM (2018)
The method developed is precise, accurate and reliable with low reagent consumption compared with conventional solid-phase extraction. The analysis time was decreased because, with the use of the microextraction plate, the step of the evaporation and reconstitution of the analyte was avoided. The method is applicable to quantify the plasma total levels (esterified + free) of 8-iso-PGF2α.
Keyphrases
- solid phase extraction
- liquid chromatography tandem mass spectrometry
- molecularly imprinted
- high performance liquid chromatography
- simultaneous determination
- tandem mass spectrometry
- gas chromatography mass spectrometry
- liquid chromatography
- high resolution
- gas chromatography
- ultra high performance liquid chromatography
- mass spectrometry
- high resolution mass spectrometry