An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation.
Jingyi ZhuJiacheng HeMichael VeranoAyoola T BrimmoAyoub GliaMohammad A QasaimehPengyu ChenJose O AlemanWeiqiang ChenPublished in: Lab on a chip (2018)
Although many advanced biosensing techniques have been proposed for cytokine profiling, there are no clinically available methods that integrate high-resolution immune cell monitoring and in situ multiplexed cytokine detection together in a biomimetic tissue microenvironment. The primary challenge arises due to the lack of suitable label-free sensing techniques and difficulty for sensor integration. In this work, we demonstrated a novel integration of a localized-surface plasmon resonance (LSPR)-based biosensor with a biomimetic microfluidic 'adipose-tissue-on-chip' platform for an in situ label-free, high-throughput and multiplexed cytokine secretion analysis of obese adipose tissue. Using our established adipose-tissue-on-chip platform, we were able to monitor the adipose tissue initiation, differentiation, and maturation and simulate the hallmark formation of crown-like structures (CLSs) during pro-inflammatory stimulation. With integrated antibody-conjugated LSPR barcode sensor arrays, our platform enables simultaneous multiplexed measurements of pro-inflammatory (IL-6 and TNF-α) and anti-inflammatory (IL-10 and IL-4) cytokines secreted by the adipocytes and macrophages. As a result, our adipose-tissue-on-chip platform is capable of identifying stage-specific cytokine secretion profiles from a complex milieu during obesity progression, highlighting its potential as a high-throughput preclinical readout for personalized obesity treatment strategies.
Keyphrases
- adipose tissue
- high throughput
- label free
- insulin resistance
- single cell
- high fat diet
- high fat diet induced
- high resolution
- metabolic syndrome
- weight loss
- type diabetes
- rheumatoid arthritis
- stem cells
- anti inflammatory
- skeletal muscle
- weight gain
- circulating tumor cells
- photodynamic therapy
- physical activity
- oxidative stress
- gold nanoparticles
- cell therapy
- body mass index