Hybrid Cyanine/Methotrexate Nanoparticles for Synergistic PDT/Chemotherapy of Breast Cancer.
Prapassara MuangsopaKantapat ChansaenpakSastiya KampaengsriJadsada SaetiewParinya NoisaPanomsak MeemonAnyanee KamkaewPublished in: ACS applied bio materials (2023)
Typically, nanomedicine was prepared using a nanocarrier to load cargo for specific purposes. In this work, a carrier-free nanosystem for imaging and photodynamic (PDT)/chemo combination therapy was developed using simple self-assembly of a dye and a chemotherapeutic agent. The resulting nanoparticles ( I 2 -IR783/MTX@NPs ) exhibited a spherical morphology with a size of 240.6 ± 2.5 nm. I 2 -IR783/MTX@NPs had substantial internalization in 4T1 murine breast cancer cells and showed a synergistic anticancer effect after NIR light irradiation. Additionally, the 3D tumor model exhibits the same phototoxicity of nanoparticles as a 2D cell culture. The PDT efficiency of the nanosystem in the physiological environment was confirmed by the detection of intracellular reactive oxygen species as well as the live/dead viability/cytotoxicity assay following NIR light exposure. In addition, optical coherence tomography (OCT) was used as an alternative tool to monitor the response after treatment. Therefore, I 2 -IR783/MTX@NPs show great potential use in theranostic application for breast cancer PDT-chemotherapy.
Keyphrases
- photodynamic therapy
- combination therapy
- cancer therapy
- fluorescence imaging
- reactive oxygen species
- optical coherence tomography
- drug delivery
- breast cancer cells
- locally advanced
- high resolution
- oxide nanoparticles
- diabetic retinopathy
- high throughput
- squamous cell carcinoma
- high dose
- highly efficient
- radiation therapy
- chemotherapy induced
- walled carbon nanotubes
- climate change
- mass spectrometry
- rectal cancer
- drug release
- quantum dots
- single cell
- fluorescent probe