A new chemical approach for proximity labelling of chromatin-associated RNAs and proteins with visible light irradiation.

Lan LiJiying LiangHao LuoK Ming Tam Edmund Chun Ming Tse Ying Li

Published in: Chemical communications (Cambridge, England) (2019)

A new nucleus-localized singlet oxygen generator was designed and synthesized. Its capability to label nucleus-localized biomolecules through spatially-restricted oxidation under visible light was validated using fluorescence confocal microscopy. Western blot and RT-qPCR were performed to verify the desirable spatial resolution through enriching chromatin-associated RNAs and proteins.

Keyphrases

visible light
dna damage
gene expression
transcription factor
single molecule
genome wide
energy transfer
south africa
oxidative stress
quantum dots