"Lighting up" fluoride: cellular imaging and zebrafish model interrogations using a simple ESIPT-based mycophenolic acid precursor-based probe.
Neha JainPrasad M SonawaneHaoyan LiuArkaprava RoychaudhuryYoungseob LeeJongkeol AnDonghyeon KimDongwook KimYunsu KimYeu-Chun KimKyung-Bin ChoHee-Sung ParkCheol-Hee KimDavid G ChurchillPublished in: The Analyst (2023)
The discovery and implementation of media that derive from bioinspired designs and bear optical readouts featuring large Stokes shifts are of continued interest to a wide variety of researchers and clinicians. Myco-F, a novel mycophenolic acid precursor-based probe features a cleavable tert -butyldimethylsiloxy group to allow for fluoride detection. Myco-F exhibits high selectivity and specificity towards F - (Stokes shift = 120 nm). All measurements were performed in complete aqueous media (LOD=0.38 μM). Myco-F enables detection of fluoride ions in living HEK293 cells and localizes in the eye region (among other regions) of the zebrafish. DFT calculations support the proposed ESIPT working photomechanism.
Keyphrases
- fluorescent probe
- living cells
- drinking water
- high resolution
- quantum dots
- induced apoptosis
- density functional theory
- loop mediated isothermal amplification
- real time pcr
- small molecule
- cell cycle arrest
- molecular dynamics
- molecular dynamics simulations
- molecular docking
- endoplasmic reticulum stress
- ionic liquid
- mass spectrometry
- signaling pathway