Primary biocompatibility tests of poly(lactide-co-glycolide)-(poly-L-orithine/fucoidan) core-shell nanocarriers.
Duanhua CaiJingqian FanShibin WangRuimin LongXia ZhouYuangang LiuPublished in: Royal Society open science (2018)
Layer-by-layer (LbL) self-assembly is the technology used in intermolecular static electricity, hydrogen bonds, covalent bonds and other polymer interactions during film assembling. This technology has been widely studied in the drug carrier field. Given their use in drug delivery systems, the biocompatibility of these potential compounds should be addressed. In this work, the primary biocompatibility of poly(lactide-co-glycolide)-(poly-L-orithine/fucoidan) [PLGA-(PLO/fucoidan)] core-shell nanoparticles (NPs) was investigated. Atomic force microscopy revealed the PLGA-(PLO/Fucoidan)4 NPs to be spherical, with a uniform size distribution and a smooth surface, and the NPs were stable in physiological saline. The residual amount of methylene chloride was further determined by headspace gas chromatography, in which the organic solvent can be volatilized during preparation. Furthermore, cell viability, acridine orange/ethidium bromide staining, haemolysis and mouse systemic toxicity were all assessed to show that PLGA-(PLO/fucoidan)4 NPs were biocompatible with cells and mice. Therefore, these NPs are expected to have potential applications in future drug delivery systems.
Keyphrases
- gas chromatography
- atomic force microscopy
- drug delivery
- drug release
- oxide nanoparticles
- mass spectrometry
- gas chromatography mass spectrometry
- tandem mass spectrometry
- induced apoptosis
- high speed
- ionic liquid
- oxidative stress
- emergency department
- high resolution mass spectrometry
- single molecule
- cancer therapy
- bone regeneration
- current status
- type diabetes
- single cell
- cell cycle arrest
- tissue engineering
- signaling pathway
- endoplasmic reticulum stress
- reduced graphene oxide
- insulin resistance
- cell death
- adverse drug
- electronic health record
- solar cells