Using RNA-seq to Assess Off-Target Effects of Antisense Oligonucleotides in Human Cell Lines.

Sven MichelKsenija SchirduanYimin ShenRichard Klar Jorg TostFrank Jaschinski

Published in: Molecular diagnosis & therapy (2020)

Avoiding suppression of off-target genes by a thorough bioinformatics screen should strongly reduce the risk for toxicities caused by antisense oligonucleotide-mediated off-target RNA suppression and finally result in safer antisense oligonucleotide-based therapeutics.

Keyphrases

rna seq
nucleic acid
single cell
endothelial cells
high throughput
gene expression
transcription factor