Determination of ITS1 haplotypes of Fritillariae Cirrhosae Bulbus by amplicon sequencing.

Hoi-Yan WuKa-Lok WongSean Tsz-Sum LawWenyang NongKwun-Tin ChanJerome Ho-Lam HuiGe LinWing-Han ChanPang-Chui Shaw

Published in: Chinese medicine (2024)

Our findings suggest that the incomplete digestion in PCR-RFLP analysis of Fritillariae Cirrhosae Bulbus is caused by the presence of ITS1 haplotypes without SmaI restriction site due to intragenomic heterogeneity.

Keyphrases

single cell
solid phase extraction
molecularly imprinted
anaerobic digestion