Recent Advances in Electrochemiluminescence Visual Biosensing and Bioimaging.
Yajuan YanLurong DingJialian DingPing ZhouBin SuPublished in: Chembiochem : a European journal of chemical biology (2024)
Electrochemiluminescence (ECL) is one of the most powerful techniques that meet the needs of analysis and detection in a variety of scenarios, because of its highly analytical sensitivity and excellent spatiotemporal controllability. ECL combined with microscopy (ECLM) offers a promising approach for quantifying and mapping a wide range of analytes. To date, ECLM has been widely used to image biological entities and processes, such as cells, subcellular structures, proteins and membrane transport properties. In this review, we first introduced the mechanisms of several classic ECL systems, then highlighted the progress of visual biosensing and bioimaging by ECLM in the last decade. Finally, the characteristics of ECLM were summarized, as well as some of the current challenges. The future research interests and potential directions for the application of ECLM were also outlooked.
Keyphrases
- label free
- high resolution
- quantum dots
- induced apoptosis
- sensitive detection
- fluorescent probe
- living cells
- climate change
- cell cycle arrest
- energy transfer
- deep learning
- high throughput
- optical coherence tomography
- oxidative stress
- high speed
- endoplasmic reticulum stress
- high density
- real time pcr
- cell proliferation