Advantages of Single-Stranded DNA Over Double-Stranded DNA Library Preparation for Capturing Cell-Free Tumor DNA in Plasma.

Jing ZhuJinyong HuangPeng ZhangQianxia LiManish KohliChiang-Ching HuangLiang Wang

Published in: Molecular diagnosis & therapy (2021)

ssDNA libraries preserve more diversity and capture more ctDNA than dsDNA libraries. The ssDNA library method is preferred when performing genomic analysis of ctDNA.

Keyphrases

circulating tumor
cell free
circulating tumor cells
nucleic acid
single molecule
molecularly imprinted
mass spectrometry
high resolution