Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine.
Kamlesh PatelMarcos Fernandez-VillamarinCraig WardJanet M LordPeter TinoPaula M MendesPublished in: The Analyst (2022)
The kynurenine metabolite is associated with many diseases and disorders, ranging from diabetes and sepsis to more recently COVID-19. Here we report a fluorescence-based assay for the detection of kynurenine in urine using a specific chemosensor, 3-formyl-4-(ethylthio)-7-(diethylamino)-coumarin. The assay produces a linear response at clinically relevant ranges (1-20 μM), with a limit of detection of 0.7 μM. The average standard addition recoveries of kynurenine in synthetic urine samples are near to 100%, and the relative standard deviation values are less than 8%. The established fluorescence assay for quantitative analysis of kynurenine in urine is facile, sensitive and accurate and holds great potential for low-cost and high-throughput analysis of kynurenine in clinical laboratory settings.
Keyphrases
- high throughput
- low cost
- single molecule
- high resolution
- coronavirus disease
- sars cov
- cardiovascular disease
- energy transfer
- loop mediated isothermal amplification
- adipose tissue
- insulin resistance
- risk assessment
- highly efficient
- fluorescent probe
- skeletal muscle
- septic shock
- reduced graphene oxide
- aqueous solution
- sensitive detection