Reshaping the active pocket of promiscuous lactonases for degrading bulky organophosphate flame retardants.
Yuanyuan ZhangXiaoyan ZhangHuan ChengMario Roque Huanca NinaJun GeYun-Peng BaiPublished in: Chemical communications (Cambridge, England) (2021)
Engineering of a promiscuous lactonase via semi-rational evolution gave a 1007-fold improvement in its catalytic activity in the degradation of triphenyl phosphate (TPHP). TPHP is a typical bulky organophosphate flame retardant (OPFR) and is widely used in industry. To the best of our knowledge, this is the first artificial enzyme capable of degrading OPFRs.