An adaptive optics module for deep tissue multiphoton imaging in vivo.
Cristina RodríguezIchun Anderson ChenJosé A RiveraManuel Alexander MohrYajie LiangRyan G NatanWenzhi SunDaniel E MilkieThomas G BifanoXiaoke ChenNa JiPublished in: Nature methods (2021)
Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.
Keyphrases
- high resolution
- spinal cord
- mass spectrometry
- living cells
- spinal cord injury
- high speed
- tandem mass spectrometry
- single molecule
- multiple sclerosis
- white matter
- endothelial cells
- gram negative
- oxidative stress
- brain injury
- copy number
- gene expression
- drug induced
- fluorescent probe
- photodynamic therapy
- low cost
- energy transfer