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An adaptive optics module for deep tissue multiphoton imaging in vivo.

Cristina RodríguezIchun Anderson ChenJosé A RiveraManuel Alexander MohrYajie LiangRyan G NatanWenzhi SunDaniel E MilkieThomas G BifanoXiaoke ChenNa Ji
Published in: Nature methods (2021)
Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.
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