MiR-3175 promotes epithelial-mesenchymal transition by targeting Smad7 in human conjunctiva and pterygium.
Xincao ZhongJinlong TangHongyi LiXin ShiYihua WuDajing XiaHuina ZhangJuan YeHan WuPublished in: FEBS letters (2019)
MicroRNAs (miRNAs) are involved in the post-transcriptional regulation of mRNAs. However, the function of miRNAs is unclear in epithelial-mesenchymal transition (EMT) of pterygium, a disease characterized by abnormal fibrovascular proliferation and invasion on the ocular surface. Since MiR-3175 is upregulated in pterygium tissues compared to normal conjunctival tissues, in this study, we investigated the role of MiR-3175 in EMT of a cultured human conjunctival epithelium cell line (CCL) and further validated the findings in pterygium tissues from patients. Our study demonstrates that MiR-3175 promotes proliferation, migration, invasion, and EMT of CCL cells by directly inhibiting Smad7. In conclusion, our data suggested that the MiR-3175-Smad7 axis may play a critical role in pathogenesis and development of pterygium.
Keyphrases
- epithelial mesenchymal transition
- cell proliferation
- transforming growth factor
- long non coding rna
- signaling pathway
- long noncoding rna
- endothelial cells
- induced apoptosis
- gene expression
- newly diagnosed
- ejection fraction
- pi k akt
- prognostic factors
- electronic health record
- liver injury
- mass spectrometry
- patient reported outcomes
- data analysis
- high speed