Identification of enzymatic genes with the potential to reduce biomass recalcitrance through lignin manipulation in Arabidopsis.

Shingo SakamotoNaofumi KamimuraYosuke TokueMiyuki T NakataMasanobu YamamotoShi HuEiji MasaiNobutaka MitsudaShinya Kajita

Published in: Biotechnology for biofuels (2020)

Simple colorimetric characterization of stem lignin is useful for simultaneous screening of many genes with the potential to reduce lignin recalcitrance. In addition to F5H, the positive control, we identified three enzyme-coding genes that can function as genetic tools for lignin manipulation. Two of these genes (calB and couA) accelerate sugar release from transgenic lignocelluloses.

Keyphrases

genome wide
bioinformatics analysis
ionic liquid
genome wide identification
transcription factor
hydrogen peroxide
gold nanoparticles
dna methylation
gene expression
risk assessment
climate change
human health
sensitive detection
living cells
cell wall