Unravelling the Helianthus tuberosus L. (Jerusalem Artichoke, Kiku-Imo) Tuber Proteome by Label-Free Quantitative Proteomics.
Ranjith Kumar BakkuRavi GuptaCheol Woo MinSun-Tae KimGenboku TakahashiJunko ShibatoSeiji ShiodaFumiko TakenoyaGanesh Kumar AgrawalRandeep RakwalPublished in: Molecules (Basel, Switzerland) (2022)
The present research investigates the tuber proteome of the 'medicinal' plant Jerusalem artichoke (abbreviated as JA) ( Helianthus tuberosus L.) using a high-throughput proteomics technique. Although JA has been historically known to the Native Americans, it was introduced to Europe in the late 19th century and later spread to Japan (referred to as 'kiku-imo') as a folk remedy for diabetes. Genboku Takahashi research group has been working on the cultivation and utilization of kiku-imo tuber as a traditional/alternative medicine in daily life and researched on the lowering of blood sugar level, HbA1c, etc., in human subjects (unpublished data). Understanding the protein components of the tuber may shed light on its healing properties, especially related to diabetes. Using three commercially processed JA tuber products (dried powder and dried chips) we performed total protein extraction on the powdered samples using a label-free quantitate proteomic approach (mass spectrometry) and catalogued for the first time a comprehensive protein list for the JA tuber. A total of 2967 protein groups were identified, statistically analyzed, and further categorized into different protein classes using bioinformatics techniques. We discussed the association of these proteins to health and disease regulatory metabolism. Data are available via ProteomeXchange with identifier PXD030744.
Keyphrases
- label free
- mass spectrometry
- high throughput
- type diabetes
- protein protein
- cardiovascular disease
- binding protein
- endothelial cells
- public health
- physical activity
- electronic health record
- machine learning
- transcription factor
- liquid chromatography
- climate change
- small molecule
- metabolic syndrome
- adipose tissue
- insulin resistance
- weight loss
- high performance liquid chromatography
- simultaneous determination
- drug induced