Fermentative Biohydrogen and Biomethane Production from High-Strength Industrial Food Waste Hydrolysate Using Suspended Cell Techniques.

The food waste was very difficult to treat in a proper way since its high-organic matter. The novel biohythane (H 2  + CH 4 ) production from high-strength industry food waste hydrolysate in two steps anaerobic well mixed batch bioreactor was carried out in this study using cultivated microflora. The temperature was controlled at 37 °C and initial substrate concentration of industrial food waste hydrolysate varied from 60, 80, 100, and 120 g COD/L, respectively. The pH, TS, VS, and SCOD were analyzed from the influent and effluent samples. These analytical parameters showed the correlations between the biogas production rates and yields in the batch fermentation system. This study was the first time to use the industry food waste hydrolysate which was collected from the subcritical water hydrolysis process. In this study, the optimal biohydrogen and biomethane yield production by using suspended cells were 0.65 mL H 2 /g COD and 203.72 mL CH 4 /g COD where the initial substrate concentrations of total COD and SCOD were 60 g/L and 39.80 g/L, respectively. The optimal of the biohydrogen and biomethane yields production by using suspended cells were 0.65 mL H 2 /g COD and 203.72 mL CH 4 /g COD where the initial substrate concentrations of total COD and SCOD were 60 g/L and 39.80 g/L, respectively. The results of this study supported that the cultivation of inoculum in a suspended cell type can have a higher tolerance for the biohydrogen and biomethane production in a high-strength initial substrate concentration of 60 g COD/L.