Dichloroisocoumarins with Potential Anti-Inflammatory Activity from the Mangrove Endophytic Fungus Ascomycota sp. CYSK-4.
Yan ChenZhaoming LiuHongju LiuYahong PanJing LiLan LiuZhigang ShePublished in: Marine drugs (2018)
Three new isocoumarins-dichlorodiaportintone (1), desmethyldichlorodiaportintone (2) and desmethyldichlorodiaportinol (3)-as well as six known analogues (4-9) were isolated from the culture of the mangrove endophytic fungus Ascomycota sp. CYSK-4 from Pluchea indica. Their structures were elucidated by analysis of spectroscopic data. The absolute configuration of compounds 1 and 2 were determined by the modified Mosher's method. Compound 2 showed significant anti-inflammatory activity by inhibiting the production of NO in LPS-induced RAW 264.7 cells with IC50 value of 15.8 μM, while compounds 1, 5, and 6 exhibited weak activities with IC50 values of 41.5, 33.6, and 67.2 μM, respectively. In addition, compounds 1, 5, and 6 showed antibacterial effects against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter calcoaceticus with the MIC values in the range of 25-50 μg·mL-1.
Keyphrases
- klebsiella pneumoniae
- escherichia coli
- lps induced
- bacillus subtilis
- staphylococcus aureus
- multidrug resistant
- inflammatory response
- molecular docking
- induced apoptosis
- biofilm formation
- signaling pathway
- cell cycle arrest
- high resolution
- electronic health record
- acinetobacter baumannii
- drug resistant
- endoplasmic reticulum stress
- cell death
- machine learning
- oxidative stress
- risk assessment
- methicillin resistant staphylococcus aureus
- molecular dynamics simulations
- artificial intelligence