Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model.
Shinji KumegawaGen YamadaDaiki HashimotoTsuyoshi HirashimaMizuki KajimotoKyoichi IsonoKota FujimotoKentaro SuzukiKazuhisa UemuraMasatsugu EmaShinichi AsamuraPublished in: Biological procedures online (2021)
The current surgical procedures and analysis on the post-surgical status are the first case to observe vasculatures in vivo with a transparent sheet. Systematic analyses including the FITC-dextran mediated snap shot images observation suggest the elongation of fine (neo) lymphatic vasculature. Post-surgical analyses including LSFM and 3D histological structural reconstruction, are suitable to reveal the fixed structures of blood and lymphatic vessels formation.