In summary, Meta-Prism 2.0 has changed the resource-intensive sample search scheme to an effective procedure, which could be conducted by researchers every day even on a laptop, for insightful sample search, similarity analysis, and knowledge discovery. Meta-Prism 2.0 can be accessed at https://github.com/HUST-NingKang-Lab/Meta-Prism-2.0, and the web server can be accessed at https://hust-ningkang-lab.github.io/Meta-Prism-2.0/.