Detecting Mycobacterium Tuberculosis using a nitrofuranyl calanolide-trehalose probe based on nitroreductase Rv2466c.
Xueyuan LiPengfei GengXiaoqiao HongZhaogang SunGang LiuPublished in: Chemical communications (Cambridge, England) (2021)
A new Mtb fluorescent probe, NFC-Tre-5, was reported that could label single cells of Mtb under various stress conditions via a unique fluorescence off-on feature by a Rv2466c-mediated reductive mechanism. This probe effectively facilitates the rapid and specific detection of Mtb in the host cell during infection and the detection of Mtb in sputum samples from patients.
Keyphrases
- mycobacterium tuberculosis
- living cells
- pulmonary tuberculosis
- fluorescent probe
- loop mediated isothermal amplification
- end stage renal disease
- ejection fraction
- single molecule
- newly diagnosed
- induced apoptosis
- quantum dots
- chronic kidney disease
- label free
- real time pcr
- single cell
- cell therapy
- deep learning
- cystic fibrosis
- patient reported outcomes
- cell death
- pi k akt